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. 2016 Apr 18;7(24):36220–36234. doi: 10.18632/oncotarget.8811

Figure 2. BMI1 transcriptionally regulates miR-200c/141 promoter.

Figure 2

A. 293T cells were transiently transfected with miR-200c/141 promoter-reporter (pGL4.8-PmiR-200c/141) and pTK-Luc plasmids with HA-BMI1 or pRS-shBMI1 plasmids, and normalized luciferase activity was determined after 48 hrs and plotted. B. Binding of BMI1 to miR-200c/141 promoter was studied by ChIP assay. Four different sets of primers covering 683 bp of miR-200c/141 promoter region were designed to amplify BMI1 immunoprecipitated chromatin and the qPCR was performed as described in the Experimental Methods. The approximate position of E-box 2, Z-Box 1, E-Box 3, and Z-box 2 are indicated in the promoter region. Error bars represent ±S.D, * p <0.05.