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. 2016 Apr 18;7(24):36220–36234. doi: 10.18632/oncotarget.8811

Figure 6. PTC-209 induces premature cellular senescence in human cells.

Figure 6

A. Western blot analysis of BMI1, H2AK119Ub, total H2A and β-actin and molecular markers of senescence (p53, p21, pRB and p16), in MRC5 cells. The cells were mock treated or treated for 48 hrs with PTC-209. B. MRC5, MDA-MB-231 and MCF7 cells (as indicated) were treated with PTC-209 for 48 hrs and co-stained with SA-β-gal and EdU to determine the induction of premature senescence by PTC-209. The number of positive cells were counted in 4 random fields and % positive cells for each marker plotted as shown. Error bars represent ±S.D, * p <0.05.