Figure 4. Forced Sirt1 expression partly abolished the effect of miR-133b on cell proliferation and invasion.

(A) Western blot was performed to examine the Sirt1 protein levels in U87 cells transfected with miR-133b mimic, or co-transfected with miR-133b mimic and Sirt1-overexpressing plasmid (miR-133b + Sirt1). (B) MTT assay and (C) transwell assay were conducted to determine the cell proliferation and invasion in each group. **P < 0.01 vs. miR-133b. bar = 100 μm.