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. 2016 Apr 22;7(24):36338–36352. doi: 10.18632/oncotarget.8916

Figure 6. ACL and PDK knock-down increase pyruvate levels and affect PDH activity.

Figure 6

MIR126 transfected Met5A, H28 and IstMes2 cells and their empty plasmid-transfected counterparts were analyzed for PDH activity A. and pyruvate levels B. H28 cells were selected to stably express the empty vector (H28pRS) or the ACL shRNA construct (H28ACL, insert), and PDH activity C. and pyruvate levels D. evaluated in the presence or absence of PDK inhibitor DCA (20 mM). The data shown are mean values ± S.D. derived from three independent experiments. Comparisons among groups were determined by one-way ANOVA with Tukey post-hoc analysis; the symbol “*” indicates significant differences compared with empty plasmid-transfected cells, and the symbol “°” indicates significance between DCA treated and untreated cells.