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. 2016 Apr 27;7(24):36501–36509. doi: 10.18632/oncotarget.9038

Figure 5. BRG1 regulation of STAT3/VEGFC signaling mediates lymphangiogenesis.

Figure 5

A. Representative images and quantifications B. of tube formation of human lymphatic endothelial cells (HLECs) cultured with conditioned medium derived from LoVo cells. DMSO was used as vehiche control. Scale bars: 50μm. C. Immunoprecipitation using anti-BRG1 or anti-STAT3 antibodies was performed in LoVo cells, followed by immunoblotting with the indicated antibodies. D. (Left) Western blot analysis of BRG1, STAT3, p-STAT3 expression after transfection with siNC and siBRG1 in the presence or absence of S3I-201 (STAT3-specific inhibitor) in SW480 cells. (Right) Quantitative expression analysis of VEGFC protein levels by ELISA in the supernatants of SW480 cells after transfection with siNC and siBRG1 in the presence or absence of S3I-201 (STAT3-specific inhibitor) in SW480 cells. *p<0.05, **p<0.01. E. The luciferase activity fold change of STAT3 pathway reporter in LoVo and SW480 cells.