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. 2016 Apr 28;7(24):36551–36562. doi: 10.18632/oncotarget.9070

Figure 2. ZFP91 upregulates HIF-1α in colon cancer cells.

Figure 2

A, B. HCT116 and KM12C cells transfected with Flag-ZFP91 or ZFP91 siRNA were stimulated with hypoxia for final 2 h. The protein and mRNA expression levels of HIF-1α and ZFP91 were determined by western blot and RT-PCR, respectively. C, D. HCT116 and KM12C cells transfected with Flag-ZFP91 or ZFP91 siRNA together with HIF-1α promoter reporter construct pGL-WT-HIF-1α were stimulated with hypoxia for final 2 h, and then the cell lysates were subject to the measurement of dual luciferase activity. Data represented as mean ± standard deviation of three independent experiments. (**p<0.01, ***p<0.001,##p<0.01,###p<0.001). E, F. HCT116 and KM12C cells pre-treated with PCMV, Flag-ZFP91 or HA-HIF-1α together with control siRNA or HIF-1α siRNA stimulated with hypoxia for final 2 h. The cell lysates were analyzed using indicated antibodies. The mRNA expression levels of HIF-1α VEGF and EPO were determined by RT-PCR.