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. 2016 Apr 28;7(24):36551–36562. doi: 10.18632/oncotarget.9070

Figure 4. ZFP91 enhances the proliferation of colon cancer cells through HIF-1α in culture.

Figure 4

A, B. HCT116 (A) and KM12C (B) cells pre-treated with PCMV, Flag-ZFP91 or HA-HIF-1α together with control siRNA or HIF-1α siRNA, and subjected to MTT assay on days 1, 2, 3 and 4 under normoxic conditions. Data represented as mean ± standard deviation of three independent experiments (*p<0.05, **p<0.01). C, D. HCT116 and KM12C cells pre-treated with PCMV, Flag-ZFP91 or HA-HIF-1α together with control siRNA or HIF-1α siRNA under normoxic conditions, and then cells proliferation was measured by colony formation assay (C) and EdU assay (D). Data represented as mean ± standard deviation of three independent experiments (*p<0.05, **p<0.01). E. Flow cytometry analysis was performed to detect cell cycle of HCT116 and KM12C cells pre-treated with PCMV, Flag-ZFP91 or HA-HIF-1α together with control siRNA or HIF-1α siRNA under normoxic conditions.