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. 2016 May 2;7(24):36655–36665. doi: 10.18632/oncotarget.9131

Figure 1. Downregulation of FGF9 by siRNA in GC cells inhibits cell growth and induces apoptosis.

Figure 1

Growth of MGC-803 A. and SGC-7901 cells B. transfected with FGF9 siRNA or control. MGC-803 cells transfected with FGF9 siRNA or control were analysed by colony formation assay C. MGC-803 cells transfected with FGF9 siRNA or control were analysed by a histogram (*P < 0.001) D. MGC-803 (upper layer) and SGC-7901 (below layer) cells were transfected with FGF9 siRNA (right figure) or control (left figure) and the expression of FGF9 was detected by immunofluorescence staining E. MGC-803 cells were transfected with FGF9 siRNA or control and apoptotic cells were evaluated by Annexin V-FITC and PI staining and FACS F. Apoptotic morphological analysis of MGC-803 cells transfected with FGF9 siRNA or control by DAPI staining G. All data are presented as mean±s.e.m from at least three separate experiments.