The relative RNA amounts were normalized to β-actin mRNA. The primer sequences were seen as below.
Gene | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
MUC16 | AGCACCCAGCACTTCTACCT | GCGCATCCTCAATATTCCTT |
Vimentin | GAGAACTTTGCCGTTGAAGC | GCTTCCTGTAGGTGGCAATC |
E-Cadherin | CCCATCAGCTGCCCAGAAAATGAA | CTGTCACCTTCAGCCATCCTGTTT |
N-cadherin | CCTTTCAAACACAGCCACGG | TGTTTGGGTCGGTCTGGATG |
Fibronctin | CAGTGGGAGACCTCGAGAAG | TCCCTCGGAACATCAGAAAC |
Cyclin D1 | CCGTCCATGCGGAAGATC | GAAGACCTCCTCCTCGCACT |
Survivin | ACCACCGCATCTCTAC | TCCTCTATGGGGTCGT |
Axin2 | GCAAACTTTCGCCAACCGTG | CTCTGGAGCTGTTTCTTACTGCCC |
c-Myc | CCCTTGCCGCATCCACG | CGAGGTCATAGTTCCTGTTGGTG |
Snail | GACCCCAATCGGAAGCCTAACTA | AGCCTTTCCCACTGTCCTCATCT |
β-actin | TCCCTGGAGAAGAGCTACG | GTAGTTTCGTGGATGCCACA |