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. 2016 May 5;7(24):36800–36813. doi: 10.18632/oncotarget.9191

The relative RNA amounts were normalized to β-actin mRNA. The primer sequences were seen as below.

Gene Forward (5′-3′) Reverse (5′-3′)
MUC16 AGCACCCAGCACTTCTACCT GCGCATCCTCAATATTCCTT
Vimentin GAGAACTTTGCCGTTGAAGC GCTTCCTGTAGGTGGCAATC
E-Cadherin CCCATCAGCTGCCCAGAAAATGAA CTGTCACCTTCAGCCATCCTGTTT
N-cadherin CCTTTCAAACACAGCCACGG TGTTTGGGTCGGTCTGGATG
Fibronctin CAGTGGGAGACCTCGAGAAG TCCCTCGGAACATCAGAAAC
Cyclin D1 CCGTCCATGCGGAAGATC GAAGACCTCCTCCTCGCACT
Survivin ACCACCGCATCTCTAC TCCTCTATGGGGTCGT
Axin2 GCAAACTTTCGCCAACCGTG CTCTGGAGCTGTTTCTTACTGCCC
c-Myc CCCTTGCCGCATCCACG CGAGGTCATAGTTCCTGTTGGTG
Snail GACCCCAATCGGAAGCCTAACTA AGCCTTTCCCACTGTCCTCATCT
β-actin TCCCTGGAGAAGAGCTACG GTAGTTTCGTGGATGCCACA