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. 2016 May 5;7(24):36814–36828. doi: 10.18632/oncotarget.9192

Figure 3. Androgen/AR axis stimulates Nanog expression and promotes HCC cells stemness.

Figure 3

A. Level of Nanog mRNA was detected at the different time points after treatment with DHT together with or without ASC-J9 in T1224+1 and Huh7+7 cells. Values are normalized to GAPDH and represented the mean ± SD of triplicate samples. B. Protein levels of AR and NANOG expression was measured in NanogPos and NanogNeg cells from T1224+1 and Huh7+7after treatment with DHT and with or without ASC-J9 for 24h. C-D. Clone and Sphere formation efficiencies of NanogPos and NanogNeg cells from T1224+1 and Huh7+7 after treated with DHT, or with or without ASC-J9. Data was presented as means ± SD of three independent experiments. The clone and sphere formation efficiency were calculated as the percentage of cell clones or spheres number divided the total cells number that seed in each well. Scale bars, 200μm, p<0.05(*), p<0.01(**) and p<0.001(***).