Skip to main content
. 2016 May 6;7(24):36842–36853. doi: 10.18632/oncotarget.9209

Figure 2. CCL7 increases expression of chemokine receptor CCR3.

Figure 2

A. CCL7 overexpression induces morphological changes in HCT116 cells. Representative images of cells taken at 400× magnification are shown. B. Total cell lysates were subjected to western blot analysis to confirm CCL7 overexpression. Actin was used as a loading control. C. Transcriptional levels of CCL7 were measured using real-time PCR. β-actin expression was used as an internal control to obtain the relative quantification of gene expression. D. CCL7 secretion was measured by multiplex magnetic immunoassay of HCT116 cell lysates and supernatants. Expression patterns of CCR1, -2, -3, and -5 protein were monitored with E. Western blot and F, G. FACS analysis in CCL7 overexpressing (E, F) or CCL7 recombinant protein treated HCT116 cells (G). Columns: means ± SEs. **P < 0.01; ***P < 0.001.