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. 2016 May 6;7(24):36842–36853. doi: 10.18632/oncotarget.9209

Figure 4. Involvement of CCR3 in CCL7-induced ERK and JNK activation.

Figure 4

A. Protein phosphorylation array in HCT116 cells stably transfected with GFP/CCL7 with or without treatment with 20 nM SB 328437 (CCR3 inhibitor). B. Expression of phosphorylated and total ERK/JNK in negative siRNA control-treated or CCL7 specific siRNA-treated HCT116 cell. C. Western blot analysis of phosphorylated and total ERK/JNK in HCT116 cells stably transfected with GFP/CCL7 with or without treatment with 20 nM SB 328437. D. Expression of phosphorylated and total ERK/JNK and EMT markers in HCT116 cells stably transfected with GFP or CCL7 with or without treatment with 20 uM U0126 (ERK/JNK inhibitor) was measured by western blotting. Actin was used as a loading control.