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. 2016 May 9;7(24):36940–36955. doi: 10.18632/oncotarget.9230

Figure 1. Estradiol (E2) mediates levels of certain miRNAs, and miR-124 is the most prominently downregulated miRNA which is inhibited by E2 treatment in estrogen receptor (ER) positive BC cells.

Figure 1

A. MCF7, the ER-positive BC cells were cultured estrogen-free medium for 72 h, then treated with 10 nM E2 or equal amount of ethyl alcohol (Eth) as solvent control. The expression levels of miRNAs were analyzed by qRT-PCR and U6 levels were used as internal control, and normalized to the values of Eth control. Data were presented as the means ± SD from three independent experiments with triple replicates per experiment. * indicates significant difference upon E2 treatment at P < 0.05. B. E2 treatment reduced miR-124 expression in MCF7 cells. Cells were cultured with E2 or Eth for 0, 6, 12 and 24 h. The relative miR-124 expression levels were analyzed as above. Data were presented as the means ± SD from three independent experiments with triple replicates per experiment. * and ** indicate significant difference under E2 treatment when compared to solvent control Eth with P < 0.05 and P < 0.01, respectively. C. E2 treatment had no effect on miR-124 expression in MDA-MB-231 cells. ER-negative BC cells MDA-MB-231 were treated and miR-124 was detected as above.