Figure 3. Downregulation of endogenous miR-124 promotes the proliferation, migration and invasion of BC cells.

A. and B. MCF7 and MDA-MB-231 cells were transfected with anti-miR-124 inhibitor (Anti-miR-124), or control anti-sense RNA (anti-miR-NC). Cell Counting Kit-8 (CCK-8) was used to detect cell vitality every 24 h, and the results were presented as the means± SD from three independent experiments performed in quintuple. * and ** indicate significant difference compared to anti-miR-NC with P < 0.05 and P < 0.01, respectively. C. and D. The treated cells were used to perform wound healing assay to estimate the ability of cell migration. A sterile 10 μl pipette tip was used to scratch the cells to form a wound when the different treated cells were cultured to 90% confluence. The wound gaps were photographed (top) and measured (bottom). Data were presented as the means±SD. ** indicates significant difference between two groups at P < 0.01 E. and F. The cells above were used to perform Matrigel invasion assay as described. After incubation for 24 h, the cells in the bottom of the invasion chamber were photographed and the acetic acid-eluted solution was quantified using a standard microplate reader at 570 nm. Data were presented as the means ± SD from three independent experiments with triple replicates per experiment. ** indicates significant difference between two groups at P < 0.01.