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. 2016 May 18;7(24):37030–37042. doi: 10.18632/oncotarget.9458

Figure 4. Forced expression of survivin blocks TAT-IL-24-KDEL-induced apoptosis in cancer cells.

Figure 4

(A) Human lung tumor cells H460 were stably transfected with the pcDNA3.1/survivin plasmid (H460/survivin) or the pcDNA3.1 vector alone (H460/neo). Survivin expression was determined by western blot. (B) Survivin overexpression increases clonigenic cell survival after TAT-IL-24-KDEL treatment. (C) Lysates were prepared from H460/survivin or H460/neo cells treated with various concentrations of TAT-IL-24-KDEL for 48 h, and western blot was performed to monitor survivin protein levels and cleavage of PARP. Percentage of apoptosis was determined by flow cytometry. (D) H460/neo and H460/survivin cells were treated with 50 nM TAT-IL-24-KDEL for 48 h. The cytosolic release of pro-apoptotic proteins Cytochrome c and Smac/Diablo was determined by western blot. The cleavage of DFF45 completely blocked in H460/survivin cells. (E) H460/neo and H460/survivin cells were treated with various concentrations of TAT-IL-24-KDEL for 48 h. Each bar represents the mean ± SE (n = 5) caspase-3/7 activity. All values were normalized to cell number and expressed as the fold increase over the control (*P < 0.05; **P < 0.01 versus PBS-treated group).