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. 2016 May 11;7(24):37177–37191. doi: 10.18632/oncotarget.9287

Figure 4. MDM2 knockdown promotes MET in MDA-MB-231 cells.

Figure 4

MDM2-siRNA-5 had the highest inhibition efficiency and MDM2-siRNA-1 had the second highest inhibition efficiency A. Knockdown of MDM2 in MDA-MB-231 cells induced morphological changes to an epithelial phenotype (200×) B. In 231-MDM2-siRNA-5 cells, qRT-PCR analyses showed increased E-cadherin and decreased N-cadherin, Vimentin and Snail mRNA levels, compared with the control cells (231-MDM2-NC) C. In 231-MDM2-siRNA-5 cells, western blotting showed decreased N-cadherin, Vimentin, Snail and increased E-cadherin protein levels D. ***P<0.001 and ****P<0.0001. The results are from three independent experiments. Error bars indicate the standard deviation.