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. 2016 Nov 4;7:484. doi: 10.3389/fimmu.2016.00484

Figure 2.

Figure 2

LPS-O128 and LPS-PA induce NETosis when present above a threshold value. (A) LPS-O128 and LPS-PA induce NET release only at high LPS concentrations of 8 pg LPS per neutrophil (PMN). (B) Priming of neutrophils for 1 h with recombinant TNF-α (10 ng/ml), IL-6 (10 ng/ml), IFN-α (100 ng/ml), or a mixture of all, does not promote NET release nor prime neutrophils for LPS-induced NETosis by LPS-O111 (bottom left), LPS-O128 (top middle), and LPS-PA (bottom middle) at a concentration of 6 pg LPS per neutrophil (PMN). In these graphs, NETosis induced by LPS-O128 and LPS-PA at 8 pg LPS per neutrophil is shown in dark as positive controls. Cytokines do not enhance NETosis by LPS-O128 or LPS-PA at LPS concentrations of 8 pg LPS per neutrophil (top and bottom right). Quantifications of NET release were performed by fluorometry, as outlined. *p < 0.05, **p < 0.01, and ***p < 0.001, when compared to control. Data represent mean values ± SEM of at least three experiments.