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Neutral ceramidase‐Exos have a higher NCDase activity than Con‐Exos. Exosomes from INS‐1 cells transfected with pEGFP‐C3‐NCDase (NCDase‐Exo) had higher NCDase activity compared with the exosomes from INS‐1 cells transfected with the control vector pEGFP‐C3 (Con‐Exo), # vs. *: P < 0.05. NCDase activity in NCDase‐Exos and Con‐Exos was determined by HPLC and data from triplicate experiments are expressed as fold of control (mean ± SD).
