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. 2016 Oct;13(123):20160677. doi: 10.1098/rsif.2016.0677

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Plots of the CH₂ symmetric stretch intensities (integrated over 2845–2852 cm⁻¹ of RL spectra) from hyperspectral CARS microscopy datasets of soya bean cotyledon sections at (a) day 1, (b) day 5 and (c) day 9 post imbibition. (d) Average, normalized RL spectra from at least four different CH₂-rich ROIs in the respective images show the local composition of oil bodies with same colour scheme as in (a). The spectrum of refined soya bean oil is shown in black. Grey rectangles show specific vibrational bands, and the orange rectangle marks the division between the fingerprint to CH regions of the graph. (e) Lipid unsaturation, as indicated by where A is the peak area determined from multi-peak fitting, increased from day 1 to day 5, and dropped below the starting value at day 9. (f) Lipid chain order , where and are the RL spectral intensities from 2885 and 2848 cm⁻¹, respectively, increased from approximately 0.94 at day 1 to 1.13 at day 5 before decreasing again to 1.02 by day 9. (g) Protein content, as assessed by dropped at day 9 following an increase at day 5 when compared with day 1. Error bars in (e–g) are standard deviation calculated from N > 4 spectra from ROIs within each sample. ROIs always consisted of at least 10 voxels (spectra) for averaging. Scale bar in (a–c) is 10 µm.

Inline graphic — Plots of the CH₂ symmetric stretch intensities (integrated over 2845–2852 cm⁻¹ of RL spectra) from hyperspectral CARS microscopy datasets of soya bean cotyledon sections at (a) day 1, (b) day 5 and (c) day 9 post imbibition. (d) Average, normalized RL spectra from at least four different CH₂-rich ROIs in the respective images show the local composition of oil bodies with same colour scheme as in (a). The spectrum of refined soya bean oil is shown in black. Grey rectangles show specific vibrational bands, and the orange rectangle marks the division between the fingerprint to CH regions of the graph. (e) Lipid unsaturation, as indicated by where A is the peak area determined from multi-peak fitting, increased from day 1 to day 5, and dropped below the starting value at day 9. (f) Lipid chain order , where and are the RL spectral intensities from 2885 and 2848 cm⁻¹, respectively, increased from approximately 0.94 at day 1 to 1.13 at day 5 before decreasing again to 1.02 by day 9. (g) Protein content, as assessed by dropped at day 9 following an increase at day 5 when compared with day 1. Error bars in (e–g) are standard deviation calculated from N > 4 spectra from ROIs within each sample. ROIs always consisted of at least 10 voxels (spectra) for averaging. Scale bar in (a–c) is 10 µm.