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Effect of ADM2 on NO production in HTR-8/SVneo cells. 4,5-Diaminofluorescein diacetate fluorometric assay showing NO content in the HTR-8/SVneo cells treated for 2 hours with ADM2 (10−9 M to10−7 M) in presence or absence of the NO synthase inhibitor L-NAME (10−3 M). As shown, addition of L-NAME prevented ADM2-induced increase in NO formation in HTR-8/SVneo cells. *, Significant difference compared with the control cells (n = 3; P < .05).
