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. 2016 Oct 27;7:13198. doi: 10.1038/ncomms13198

Figure 3. High inflammatory cytokines and sustained inflammation associated with passive cutaneous anaphylaxis in Nedd4-2−/− mice.

Figure 3

Ears injected i.d. with IgE anti-DNP or HMEM-pipes vehicle at 24 h after i.v. injection of DNP–HSA in Cpa3-Cre; Mcl-1+/+, mast cell-deficient Cpa3-Cre; Mcl-1fl/fl, WT BMCMCs→Cpa3-Cre; Mcl-1fl/fl, or Nedd4-2−/− BMCMCs→Cpa3-Cre; Mcl-1fl/fl mice. (a) Levels of TNF, IL-6, CCL2, and CCL3 in ear lysates; (b) H&E stained cross-sections of ears; (c) Representative flow cytometric plots; and (d) Cells recovered per ear of gated populations of polymorphonuclear (PMN) leukocytes (Gr-1+F4/80−) and macrophages (Gr-1−F4/80+ and Gr-1+F4/80+) (b) *C, cartilage. Red arrowheads indicate PMNs. Scale bars: 100 μm (insets 20 μm). Percentage values in c refer to percentage of total viable cells present in the depicted section of the plot. Data (a, median with interquartile ranges) and (d, mean±s.e.m.) are pooled from two (a) or three (d) independent experiments performed, each of which gave similar results, each with 3–5 mice per group. *P<0.05, **P<0.01, ***P<0.001 for indicated comparisons (one-way analysis of variance (ANOVA) with Bonferroni (a) or Dunnett's (d) post test).