Figure 5. Loss of mast cell–Ndfip1^−/− enhances IgE-induced mediator release.

(a) Immunoblot analysis of Ndfip1 expression in WT or Ndfip1^−/− BMCMCs. (b–g) WT and Ndfip1^−/− BMCMCs sensitized with IgE anti-DNP antibody (SPE-7, 2 μg ml⁻¹) for 16 h, then stimulated with indicated concentrations of DNP–HSA for measurements of the release of (b) histamine (30 min), (c) IL-6, (d) TNF, (e) CCL2, (f) CCL3 and (g) IL-13 (c–g all 6 h). Data (mean±s.e.m.) are pooled from the three independent experiments performed, each of which gave similar results. *P<0.05, **P<0.01, ***P<0.001 for indicated comparisons (two-way analysis of variance (ANOVA) with Bonferroni post test).