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. 2016 Oct 27;7:13226. doi: 10.1038/ncomms13226

Figure 8. mTORC2 regulates epidermal differentiation during embryogenesis.

Figure 8

(a) Toluidine blue dye penetration assay of RicEKO and control embryos at E17.5 and E18.5, and the quantification of the blue stained area (n=5 embryos/genotype). (b) Representative H&E-stained back skin of embryos at E16.5 and E17.5 and quantification of epidermal thickness (n=5 embryos/genotype). (c) qRT-PCR analysis and (d) western blotting analysis of gene expression in RicEKO and control epidermis at E17.5 (n=5 embryos/genotype). Data represents mean±s.d.; non-paired t-test was used to calculate P value. *P<0.05, **P<0.01, ***P<0.001.