Figure 3.

Knockdown of FOXD3-AS1 induced S-phase arrest in glioma cells. A. FOXD3-AS1 Smart Silencer reduced the A172 and U251 cells growth compared with NC Smart Silencer group in CCK-8 assay. B. A172 and U251 cells were transfected with indicated Smart Silencer, and 24 h later, cells were collected. Cell cycle profiling was analyzed using flow cytometry. C. Protein levels of cell cycle genes (cyclin A, CDK2, p21) in A172 and U251 cells were detected by Western blot analysis. GAPDH was used as an endogenous normalizer. Data were based on at least three independent experiments and shown as mean ± SD. **P<0.01, ***P<0.001.