Figure 6.

The FOXD3 expression was significantly downregulated in FOXD3-AS1 Smart Silencer group compared with the NC Smart Silencer group. A and B. FOXD3 mRNA and protein expressions in U87, U251 and A172 cells. C. Western blot analysis showed that the expression of FOXD3 was apparently downregulated in U251 and A172 cells after transfection with FOXD3-AS1 Smart Silencer. GAPDH was used as an endogenous normalizer. Data were based on at least three independent experiments and shown as mean ± SD. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001.