Figure 4.

PLK1 silencing decreases the migration and invasion of SGC-7901 and MKN-28 cells. A. Cells transfected with the empty vector were used as control cells. The expression of PLK1 was determined western blot with PLK1 antibody. B. RT-PCR analyses showed effective down-regulation of PLK1 following siRNA transfection. C. PLK1- and control-siRNA transfected SGC-7901 and MKN-28 cells were wounded with pipette and wound closure percentage was quantified 24 h after scratch relative to that at 0 h. *P < 0.05, **P < 0.01 compared to the control cells. D. SGC-7901 and MKN-28 cells transfected with PLK1-siRNA or control siRNA were seeded in the upper chamber. After 6 h, cells invaded through the membrane were stained and counted in five random microscopic fields.