Figure 5.

PLK1 regulates EMT in gastric cancer cells. A. Overexpression of PLK1 induced EMT in SGC7901 and MKN-28 cells. SGC7901 and MKN-28 cells were infected with empty vector or PLK1, the morphological change was examined after 10 days. B. SGC7901 and MKN-28 cells were infected with empty vector or PLK1 and the expression of the indicated EMT markers was detected by western blotting. GAPDH was used as an internal standard. C. Control siRNA or siRNA against PLK1 were transfected into SGC7901 and MKN-28 cells. After 48 h post transfections, morphology of the transfected cells were examined. D. SGC7901 and MKN-28 cells were infected with control siRNA or siRNA against PLK1 and the expression of the indicated MET markers was detected by western blotting. GAPDH was used as an internal standard.