Figure 6.

PLK1 facilities invasion of gastric cancer cells through AKT. A. Western blot result shown that the phosphorylation of AKT at Ser-473 was elevated in cells transfected with pIRES2-EGFP-PLK1. Total AKT expression was used as a loading control. B. The phosphorylation of AKT at Ser-473 was perturbed in cells transfected with PLK1 siRNA plasmid. Total AKT expression was used as a loading control. C. Expression of D2AKT was confirmed by western blot with antibody against AKT, and GAPDH was used as loading control. D. Transwell assay was performed to determine the invasion of cells co-transfected with PLK1 silencing plasmid and D2AKT plasmid. E. In the presence of GSK690693, cells were incubated for 6 h, protein extracts were analyzed by western blot with antibodies against phosphorylated AKT (S473) or AKT. F. In the presence of GSK690693 (2 μM), transwell invasion assay was conducted to evaluate the cell invasiveness after transfection. Representative pictures were taken after staining with crystal violet.