Figure 4.

NAT10 is associated with an EMT phenotype in human HCC cell lines. A. Expression of E-cadherin and vimentin in HCC cell lines as determined by Western blot. GAPDH served as a loading control. Decreased levels of NAT10 were associated with an epithelial phenotype in HCC cells, while increased NAT10 levels were associated with a mesenchymal phenotype. B. Western blot of the EMT markers E-cadherin and vimentin expression following knockdown of NAT10 with NAT10-siRNA. C. Expression of E-cadherin and vimentin following treatment with the NAT10 inhibitor Remodelin as determined by Western blot. GAPDH was used as an internal reference. D. Immunofluorescence staining of HCC cell lines for E-cadherin or vimentin. Magnification is 200×. Data represent three independent experiments.