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Preparation of GFP and luciferase labeled CRC cells. A. To allow tracing tumor formation in living mice and isolation of tumor cells from mice, we transduced the CRC cells with a lentivirus carrying both luciferase and GFP reporter under the control of a CAG promoter. B. The transduced Caco-2 cells were purified based on GFP expression by flow cytometry. C. The purified GFP+ Caco-2 cells in culture. Scale bar is 20 µm.
