Figure 5.

CD262+ CRC cells induce the highest occurrence of tumor formation in serial adoptive transplantation. A. Schematic of the experiment: 30 tumor cells were isolated from s.c. tumor developed from either CTL, CD44+, CD133+ or CD262+ Caco-2 cells, and were transplanted s.c. to new NOD/SCID mice. The formation of tumor was examined by bioluminescence after 6 weeks. The formation of the tumor was first verified by bioluminescence and then confirmed by histology of the dissected out. The newly formed tumors were then dissected out and used for isolation of 30 tumor cells for the next round of transplantation. Three rounds of transplantation were performed. B. Frequency of developing tumor. C. The tumor mass. *P<0.05. N=10.