Figure 3.

miR-134 over-expression suppresses tumor growth in vivo. A, B. Scramble- and miR-134-transfected cells were injected s.c. into nude mice. Tumor volume and weight were calculated. All data are shown as mean ± SD. Both tumor volume and weight were significantly reduced by miR-134 over-expression. **P < 0.01 compared to control. C. Hematoxylin and eosin (HE) staining demonstrated tumor formation. ki-67 expression was measured by immunohistochemistry from tumors to evaluate the proliferation ability of tumor cells. ki-67 index was significantly lower in the miR-134-overexpressing cells than in the control, indicating a weak proliferation ability. **P < 0.01 compared to control.