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. 2016 Nov 4;10:255. doi: 10.3389/fncel.2016.00255

Figure 5.

Figure 5

Baclofen mediated RGCs apoptosis via GABAB receptor. (A,B) RGCs were transfected with GABAB2 siRNA2 or control siRNA before treatment with CoCl2 and baclofen for 24 h as indicated. Cell apoptosis detected by Annexin V and PI staining methods. C2, C4 quadrant (Annexin V + /PI−) indicate the percentage of necrotic or apoptotic cells, respectively. Values represent the mean ± SD of three independent experiments. **P < 0.01 vs. basal level. (C,D) RGCs stained with Hoechst nuclear stain after treatment as described above. White arrows indicate the apoptotic nuclei. Values represent the mean ± SD of three independent experiments. *P < 0.05 vs. basal level. (E–H) RGCs were transfected with GABAB2 siRNA2 or control siRNA before treatment with CoCl2 and baclofen. Expression of apoptosis related proteins (E,F) and pathway related proteins (G,H) detected by Western blotting treated with baclofen and CoCl2 for 24 h as presented. α-tubulin served as a loading control. The level of protein in each group was expressed as the value relative to the control. The data represent the mean ± SD of three independent experiments. (*p < 0.05, vs. control).