FIGURE 6.

ADM2 treatment activates beiging of primary human white adipocytes. Primary human scWAT SVCs were differentiated into adipocytes and treated with 20 nm ADM2 for 8 h. A and B, relative mRNA levels of UCP1 and adipocyte markers. C, immunofluorescence staining of UCP1 (green) with nuclei stained by Hoechst 33258 (blue) and lipid droplets stained by LipidTOX (red). D, immunoblotting analysis of UCP1 with eIF5 used as a loading control (Ctr). E and F, mitochondrial energetics analysis. E, OCR measurement curves. F, basal respiration, ATP production, and maximal respiration OCR calculated from E. The data are represented as mean ± S.E. n = 3 independent experiments (3–6 samples/experiment). Two-tailed Student's t test: *, p < 0.05 versus control; **, p < 0.01 versus control.