TABLE 1.

Substrate specificity profile of PavNMT

Alkaloid class	Alkaloid	Substrate consumeda
		%
Pavine	(±)-Pavine	7.9 ± 1.9
1-Benzylisoquinoline	(R,S)-Tetrahydropapaverine	0.10 ± 0.01
	Papaverine	NDb
	(S)-Reticuline	9.7 ± 2.6c
	(R)-Reticuline	ND
Protoberberine	(R,S)-Scoulerine	0.8 ± 0.1
	(R,S)-Stylopine	2.7 ± 0.3
Protopine	Cryptopine	ND
Aporphine	Glaucine	ND
Morphinan	Codeine	ND
Secoberberine	Canadaline	ND
Pthalideisoquinoline	Noscapine	ND
Bisbenzylisoquinoline	Berbamine	ND

^a Under standard assay conditions: 20 μm alkaloid substrate, 20 μm SAM, 100 mm Tris-HCl, pH 7.5, in a 40-μl reaction containing 2 μg of PavNMT protein and incubated for 1 h.

^b ND, not detected.

^c Specific activity: 10.6 picokatals mg protein⁻¹.