TABLE 3.
Melting transition temperatures as determined by differential scanning fluorimetry using 8-anilinonaphthalene-1-sulfonate
| Enzyme/mutant |
Tma (difference of Tm from the apo-state) |
|||
|---|---|---|---|---|
| Apo | +THP (1 mm) | +SAH (1 mm) | +1 mm SAH + 1 mm THP | |
| °C | ||||
| PavNMT | 69.8 | 71.4 (+1.6) | 72.7 (+2.9) | 75.8 (+6.0) |
| E205A | 67.5 | 69.0 (+1.5) | 70.5 (+3.0) | 73.0 (+5.5) |
| H206A | 68.7 | 70.1 (+1.4) | 70.3 (+1.6) | 72.4 (+3.7) |
| E205A/H206A | 66.6 | 68.3 (+1.7) | 68.8 (+2.2) | 70.4 (+3.8) |
| Y79A | 70.0 | 71.4 (+1.1) | 72.7 (+2.7) | 74.8 (+4.8) |
| E80A | 69.9 | 71.1 (+1.2) | 72.5 (+2.6) | 74.2 (+4.1) |
a Values are determined from the average of duplicate measurements, which typically differ by <0.2 °C.