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Relative abundance of magnoflorine pathway alkaloids and NMT transcripts in different opium poppy organs. LC-MS analysis (A) and qRT-PCR (B) were performed using alkaloids and total RNA isolated from five individual plants. For qRT-PCR analysis, the geometric mean of actin and ubiquitin was used as internal reference, and the sample with the highest ΔCt served as calibrator for each transcript. Relative transcript abundance (2−ΔΔCt) was converted to the percentage of maximum.
