FIGURE 1.

Characterization of AMPKγ2^NI and AMPKγ2^RG mice. A, schematic diagram of human and mouse AMPKγ2 protein. Mouse AMPKγ2 has three amino acid deletions, Pro-207, Ala-253, and Val-254, versus the human protein; thus, N485I and R528G correspond to N488I and R531G in human AMPKγ2. B, modified sequence of AMPKγ2^WT, AMPKγ2^NI, and AMPKγ2^RG mice. C and D, phosphorylated AMPKα (Thr-172), total AMPKα, and phospho-AMPKα (Thr-172)/total AMPKα ratio in liver and skeletal muscle of AMPKγ2^WT, AMPKγ2^NI, and AMPKγ2^RG mice at 26 weeks of age (n = 8). E and F, phosphorylated ACC in liver and skeletal muscle of AMPKγ2^WT, AMPKγ2^NI, and AMPKγ2^RG mice at 26 weeks of age (n = 8). RU, relative units. *, p ≤ 0.05; **, p ≤ 0.01. Error bars, S.E.