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HPLC analysis of the oxygen dependence of the RcaE-catalyzed formation of lumichrome. The RcaE enzymatic reactions were run for 10 min in the presence of riboflavin, FMN, RcaB, NADH, ATP, and oxygen concentrations varying from 25 to 125 μm. No lumichrome formation was observed under anaerobic conditions (pink trace). The trace of lumichrome in this reaction is a contaminant in commercial FMN and riboflavin samples.
