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Cytotoxicity and serum stability study. A, effect of different concentration of KR7 peptide on HEK293T cells, studied by MTT reduction assay (NT stands for non-treated control cells, and buffer was used as negative control). B, comparison of cytotoxic effect of insulin amyloid intermediates (black bar) and insulin-KR7 complex (1:1 molar ratio) (white bar) on HEK293T cell at different time points. C, stability of KR7 peptide in serum was checked by RP-HPLC at different time intervals.
