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. 2016 Sep 15;291(45):23578–23588. doi: 10.1074/jbc.M116.738930

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Systemic inactivation of the Alox15 gene did not impact the oxygenation degree of sperm membrane lipids. Sperm were prepared from the epididymal cauda of male Sec46Ala-Gpx4^+/−+Alox15^+/+ and Sec46Ala-Gpx4^+/−+Alox15^−/− mice, and the total membrane lipids were extracted. After alkaline hydrolysis of the ester lipids under an argon atmosphere, aliquots of the hydrolysates were analyzed by RP-HPLC for the quantification of the oxygenated and non-oxygenated PUFAs. A, representative RP-HPLC indicating the presence of hydroxylated and non-hydroxylated polyenoic fatty acids in the total membrane lipids. The chromatographic scales were calibrated by injecting known amounts of 13-HODE (hydroxylated fatty acid), arachidonic acid, and linoleic acid. LA, linoleic acid; AA, arachidonic acid; DHA, docosahexaenoic acid; DPA, docosapentaenoic acid. B, statistic evaluation of the hydroxy fatty acid/fatty acid ratios of the sperm total lipids. For each genotype, sperm from eight mice were analyzed.