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Expression of Gpx4 and Alox15 mRNA in cauda sperm of wild-type mice
Sperm were isolated from the epididymal cauda, and total RNA was extracted. Synthesis of cDNA and amplification of the mRNAs encoding for the Gpx4 isoforms and for Alox15 was carried out as described under “Materials and Methods.” mGpx4, mitochondrial Gpx4; cGpx4, cytosolic Gpx4; nGpx4, nuclear Gpx4.
| mRNA copy numbers/1000 GAPDH mRNA copies |
||||
|---|---|---|---|---|
| mGpx4 | cGpx4 | nGpx4 | Alox15 | |
| Mean ± S.D. | 4022 ± 874 | 7772 ± 1528 | 775 ± 8 | 2.0 ± 0.4 |
