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Sequences of DNA oligomers used in this study. A, 9-mer FAMdT primer-20-mer template duplex used in standing start assays; B, 13-mer FAMdT primer-39-mer template DNA duplex used in running start assays; C, 9-mer primer-20-mer template duplex used for the single nucleotide incorporation and steady-state kinetic assays; D, biotinylated 19-mer primer-20-mer template duplexes used for the LC-MS/MS analyses of primer extension products. X = dG, 7-DHP-7-deaza-dG, or DPC.
