Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 12;291(45):23589–23603. doi: 10.1074/jbc.M116.745257

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Standing start assays for replication bypass of DNA-peptide and DNA-protein cross-links by hPol η. 9-mer FAMdT primers were annealed with 20-mers containing unmodified dG (A), deaza-DHP-dG (B), or covalent cross-links to 10-mer peptide EQKLISEEDL (C), 23-mer peptide PDAQLVPGINGKAIHLVNNESSE (D), or full-sized protein AlkB (E), histone (His) H4 (F), or eGFP (G). The resulting primer-template duplexes (0.15 μm) were incubated in the presence of hPol η (0.30 μm). Reactions were initiated by the addition of a mixture of dNTPs (500 μm) and quenched at the indicated time points. Extension products were separated by 20% denaturing PAGE and visualized by fluorescence imaging. Panel H shows results for DPC to histone (His) H4 following digestion with Proteinase K.