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Single nucleotide insertion opposite unmodified guanine (dG) and the 10-mer peptide cross-linked to C7 of 7-deazaguanine (10-mer peptide) by TLS polymerase hPol η. Primer-template duplexes (50 nm) were incubated with 50 nm polymerase in the presence of 100 μm individual dNTPs. The reactions were quenched at predetermined time points (0–60 min), and the products were separated by 20% denaturing PAGE and visualized by phosphorimaging.
