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. 2016 Sep 2;291(45):23681–23692. doi: 10.1074/jbc.M116.720763

FIGURE 3.

FIGURE 3.

Effects of cingulin knockdown on thrombin-induced cytoskeletal remodeling. HPAECs transfected with cingulin-specific siRNA or nonspecific RNA (ns-RNA) were stimulated with thrombin. Live cell imaging of the cells expressing GFP-LifeAct is shown. Snapshots depict thrombin-induced actin rearrangement and cell contraction in control and cingulin-depleted cells; bar, 5 μm. Arrows indicate cell-cell interface areas. Shown are representative results of three independent experiments.