TABLE 2.
ACh EC50 values for wild-type and mutant human α3β2 and α3β4 nAChRs
EC50 values (μm) with 95% CI. The Hill slope (nH) was obtained from concentration–response curves for ACh at wild-type and mutant human α3β2 and α3β4 nAChR subtypes. ACh concentrations from 0.01 μm to 10 mm (the highest applicable concentration) were tested. All data represent mean of n = 4–8 experiments.
| nAChR/mutant | EC50 | 95% CI | nH |
|---|---|---|---|
| μm | |||
| hα3β2 | 15.6 | 10.9–22.2 | 0.7 |
| hα3β4 | 287.6 | 274.1–301.8 | 2.3 |
| hα3β2[T59K] | 14.3 | 12.5–16.5 | 1.0 |
| hα3β4[K59T] | 29.8 | 27.9–31.9 | 1.1 |
| hα3β2[S113R] | 6.0 | 5.0–7.2 | 0.9 |
| hα3β4[R113S] | 42.9 | 36.4–50.5 | 1.1 |
| hα3β4[S114Y] | 65.1 | 56.3–75.3 | 1.1 |
| hα3β4[N115D] | 140.8 | 123.8–160.0 | 1.0 |
| hα3β4[M163K+T164S] | 63.1 | 58.6–67.9 | 1.0 |
| hα3β4[P165E] | 43.9 | 38.6–50.0 | 1.2 |
| hα3β4[T166V+M169L] | 17.5 | 15.8–19.4 | 1.1 |