. 2016 Sep 19;291(45):23779–23792. doi: 10.1074/jbc.M116.730804

TABLE 5.

Contacts of α-conotoxin AuIB with hα3β2 and hα3β4 nAChRs, respectively

Contacts between nAChR and AuIB are defined as van der Waals interactions if the distance between heavy atoms of AuIB and nAChR is between 2.6 and 4 Å. Residues of the nAChR forming hydrogen bonds with AuIB are underlined. Residues that are non-conserved between α3β2 and α3β4 nAChRs, as well as AuIB residues making contact with them are shown in bold.

Residue^a	α3β2 nAChR		α3β4 nAChR
Residue^a	+^b	−^c	+^b	−^c
Ser⁴		Asp¹⁷¹		Asp¹⁷⁰
Tyr⁵	Tyr⁹³, Tyr¹⁹⁰, Asp¹⁹⁹		Tyr⁹³, Tyr¹⁹⁰, Asp¹99
Pro⁶	Trp¹⁴⁹	Trp⁵⁷, Leu¹²¹	Trp¹⁴⁹	Trp⁵⁷, Leu¹²¹
Pro⁷	Trp¹⁴⁹, Ser¹⁵⁰, Tyr¹⁹⁷		Trp¹⁴⁹, Ser¹⁵⁰, Tyr¹⁹⁷
Phe⁹		Met³⁶, Trp⁵⁷, Thr⁵⁹, Phe¹¹⁹, Leu¹²¹		Lys⁵⁹, Glu⁶¹, Leu¹¹⁹, Leu¹²¹
Ala¹⁰	Ser¹⁵⁰	Arg⁸¹, Val¹¹¹, Phe¹¹⁹	Ser¹⁵⁰	Arg⁸¹, Ile¹¹¹
Thr¹¹	Asp¹⁵², Glu¹⁹⁵, Tyr¹⁹⁷		Asp¹⁵², Glu¹⁹⁵, Tyr¹⁹⁷
Asn¹²	Cys¹⁹², Cys¹⁹³		Cys¹⁹², Cys¹⁹³
Pro¹³		Lys⁷⁹, Val¹¹¹, Phe¹¹⁹		Ile¹¹¹, Arg¹¹³
Asp¹⁴		Lys⁷⁹		Arg¹¹³

^a Only AuIB residues making direct contact with the nAChRs are listed.

^b Residues of the principal side of the binding site.

^c Residues of the complementary side of the binding site.