TABLE 2.

Comparison of the number of splenic Ly6C^hiTLR2^hi and Ly6C^loTLR2^hi monocytes from WT versus deficient mice at 7 days post-infection (means ± S.D.)

The number of monocytes in the spleen from non-infected (NI) mice was normalized as 1N for Ly6C^hi or 1n for Ly6C^lo populations (1N ≈ 4000 cells/10⁶ events; 1n ≈ 1500 cells/10⁶ events). The data are representative of two independent experiments means ± S.D. of four animals).

	Mo-Ly6ChiTLR2hi (X N)	Mo-Ly6CloTLR2hi (X n)
WT NI	1.00 ± 0.13	1.0 ± 0.9
WT I	1.18 ± 0.04a	10.0 ± 0.4b
TLR2−/− Ic	0.59 ± 0.14d	11.0 ± 0.8
TLR9−/− I	0.70 ± 0.06d	11.0 ± 1.2
IL-12−/− I	1.00 ± 0.18	5.1 ± 1.1e
Mal/TIRAP−/− I	0.41 ± 0.03e	7.2 ± 0.2d

^a p < 0.05 compared with WT, non-infected cells.

^b p < 0.001 compared with WT, non-infected cells.

^c Cells from TLR2^−/− mice were evaluated only by the interpolation (CD11c versus Ly6C).

^d p < 0.01 compared with WT, infected cells (bold type).

^e p < 0.001 compared with WT, infected cells (bold type) by the ANOVA and Bonferroni post-tests.