Figure 7.

PD-1 is dispensable for the regulation of Th17 response to M. tuberculosis. Monocytes or DCs were cocultured with autologous CD4⁺ T cells either alone or with γ-irradiated M. tuberculosis. After 18 h, PD-1 blocking mAb or isotype control mAb were added, and cultures were maintained for 5 days. (A–C) Frequency of PD-1⁺CD4⁺ T cells (mean ± SEM, n = 4) (A), frequency of CD4⁺IL-17A⁺ T cells (mean ± SEM, n = 6) (B), and amounts of IL-17A production (mean ± SEM, n = 6) (C) in the monocyte–CD4⁺ T cell cocultures. (D–F) Frequency of PD-1⁺CD4⁺ T cells (mean ± SEM, n = 4) (D), frequency of CD4⁺IL-17A⁺ T cells (mean ± SEM, n = 6) (E), and amounts of IL-17A production (mean ± SEM, n = 6) (F) in the DC–CD4⁺ T cell cocultures. *P < 0.05; **P < 0.01; ns, not significant; as determined by one-way ANOVA.